RNA
gRNA for C
Part:BBa_K2200000:Design
Designed by: Mixiao Gui Group: iGEM17_Shenzhen_SFLS (2017-07-16)
Guide RNA (gRNA) is a specific molecule that guides the Cas nuclease to a targeted dsDNA sequence.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Our sgRNA is designed to specifically bind to the mutant gene BRAF in human A375 cell line, which lets the Cas9 protein find and cut the target.
Source
The sequence is designed according to the mutant BRAF gene sequence.